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AffiniPure-VHHâ„¢ Fragment Alpaca Anti-Human IgG (H+L)

(min X Bov, Ms, Rb Sr Prot)

Conjugate Options

Target: Human
Host: Alpaca
Antibody Format: VHH Fragment
Specificity: IgG (H+L)
Minimal Cross Reactivity: Bovine, Mouse, Rabbit Serum Proteins
Conjugate: Unconjugated
Product Category: AffiniPure-VHHâ„¢ Secondary Antibodies
RRID: AB_3095428

This product is licensed for in vitro research use only. It is not a medical device and it is not intended for diagnostic or therapeutic purposes. Product cannot be resold in any format without expressed authorization from Jackson ImmunoResearch Laboratories, Inc.
Jackson ImmunoResearch Laboratories, Inc. has trademark rights to AffiniPure-VHHâ„¢.

AffiniPure-VHHâ„¢ Secondary antibodies are available conjugated to Biotin, Cy3, Rhodamine Red-X, DyLight 405 and Alexa Fluors 488, 594, 647, 680 and 790. Contact our sales team to inquire about the unconjugated format.

Code: 609-004-213

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Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule human IgG. It also reacts with the light chains of other human immunoglobulins. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with bovine, mouse and rabbit serum proteins, but it may cross-react with immunoglobulins from other species.

AffiniPure-VHH™ secondaries are polyclonal single-domain antibodies (nanobodies†) derived from hyper-immunized alpacas. 10x smaller than conventional whole IgG antibodies, <15KDa VHH fragments are perfect for imaging experiments where good penetration is necessary. AffiniPure-VHH™ secondary antibodies are cross-adsorbed for exquisite specificity against target species with minimal cross‑reactivity to other stated species, making them suitable for application in multiple labeling experiments.

†Nanobody® is a trademark of ABLYNX N.V.
Usage
Physical State: Sterile-filtered liquid

Storage: Store at 2-8°C under sterile conditions. Prepare working dilution on day of use.
Expiration date: one year from date of receipt. The expiration date may be extended if test results are acceptable for the intended use.


Purity: The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads. Fc fragments and whole IgG molecules have been removed.
Buffer: 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6
Preservative: None

Suggested Working Concentration or Dilution Range:

10-20 µg / ml

Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.

This product is for in vitro research use only. It is not a medical device and it is not intended for diagnostic or therapeutic purposes. Product cannot be resold in any format without expressed authorization from Jackson ImmunoResearch Laboratories, Inc.


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