Combining the exquisite specificity our minimally cross-reactive antibodies have always offered with the unique penetrative capabilities of the small VHH fragment format, JIR AffiniPure-VHH™ Secondary Antibodies enable high precision staining. Read our article to explore how they behave in mouse brain tissue. 

Staining brain tissue

Staining any tissue requires experimental considerations; sometimes, it’s about the potential for cross-reactivity, sometimes it’s the permeability of the reagents, and sometimes it’s about finding the right reporter molecule. When staining multiple targets, all three considerations are critical to generating results that illustrate clear, impactful, and visually appealing findings. Whether you want to determine protein expression, where it’s localized, or if it colocalizes with another molecule, JIR AffiniPure-VHH™ Secondary Antibodies can help you amplify the detail. 

Polyclonal antibodies let you amplify the detail

Polyclonal AffiniPure-VHH™ Secondary Antibodies offer excellent sensitivity through signal amplification. As seen in Figure 2, polyclonal antibodies achieve higher labeling efficiency than monoclonal antibodies by binding to multiple locations across the primary antibody, generating a brighter signal.

Min-X for exquisite differentiation

When visualizing multiple targets, cross-adsorbed antibodies allow you to build up a multicolored image without worrying about the off-target signal from cross-reacting antibodies.  AffiniPure-VHH™ Secondary Antibodies are available cross-adsorbed (min X) to commonly used species which can reduce background and enhance specificity, enabling you to experience exquisite differentiation of target proteins.

Small means better penetration

One-tenth the size of conventional antibodies, these tiny antibody fragments offer excellent tissue penetration allowing you to reduce incubations or access targets that require specialized permeabilization treatment. Nanobodies lack an Fc effector domain which allows enhanced clearance and aids live-cell imaging techniques such as Immuno-PET.

Better choice – choose from the whole spectrum

Don’t be limited by conjugate availability; when a directly conjugated antibody isn’t available, JIR AffiniPure-VHH™ Secondary Antibodies give you access to a range of dyes without compromising resolution. We offer DyLight, Alexa Fluor®, and Cyanine™ dyes covering the spectrum, so you can choose suitable conjugates for your filter set. 

References:

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