"I have used a wide variety of secondaries and Jackson ImmunoResearch has consistently been the best. The fluorophores are bright and stable and their selective (x reactivity removed) secondaries have always shown species specificity in multiple labeling."
Alexa Fluor® 647 - conjugated antibodies absorb light maximally around 651 nm and fluoresce maximally around 667 nm. They are brighter than Cy™5 in aqueous mounting media. Alexa Fluor® 647 conjugates are the best choice of far-red emitting dyes for multiple labeling detection with a confocal microscope, and are also widely used in flow cytometry. A significant advantage of long wavelength dyes such as Alexa Fluor® 647 is the low autofluorescence of biological specimens in this region of the spectrum. However, because of its peak emission at 667 nm, Alexa Fluor® 647 cannot be seen well by eye, and it cannot be excited optimally with a mercury lamp. Therefore, Alexa Fluor® 647 is most commonly visualized with a confocal microscope equipped with an appropriate laser for excitation and a far-red detector.
Alexa Fluor® 647 is a versatile dye and can be combined with different dyes for effective 4 color immunofluorescence.
Use the spectra viewer below to compare Alexa Fluor® 647 with other fluorophores, along with instrument specifics (laser/filter) for suitability in your assay.
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