"I have used a wide variety of secondaries and Jackson ImmunoResearch has consistently been the best. The fluorophores are bright and stable and their selective (x reactivity removed) secondaries have always shown species specificity in multiple labeling."
DyLight™ 405 - conjugated secondary antibodies are excited maximally at about 400 nm and fluoresce with a peak at about 421 nm. They are very bright and photostable, but their optimal use requires instruments equipped with a 405 nm laser and a 420 nm emission filter.
Use the spectra viewer below to compare DyLight 405 with other fluorophores, along with instrument specifics (laser/filter) for suitability in your assay.
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