Physical State: Sterile-filtered liquid
Storage: Store at 2-8°C. Prepare working dilution on day of use. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid.
Extended storage: Add an equal volume of glycerol (ACS grade or better) for a final concentration of 50%, and store at -20°C as a liquid.
Expiration date: one year from the date of receipt. The expiration date may be extended if test results are acceptable for the intended use.
Buffer: 0.01M Tris-HCl, 0.25M NaCl, pH 8.0
Stabilizer: 30 mg/ml Bovine Serum Albumin (IgG-Free, Protease-Free)
Preservative: 0.05% Sodium Azide
Suggested Working Concentration or Dilution Range:
ELISA:- 1-2 µg / ml
Western Blot:- 0.1-1 µg / ml
Histo-/Cyto-Chemistry:- 1-2 µg / ml
Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.