"I have used a wide variety of secondaries and Jackson ImmunoResearch has consistently been the best. The fluorophores are bright and stable and their selective (x reactivity removed) secondaries have always shown species specificity in multiple labeling."
Janet Duerr, Ohio UniversityRating: 5.0
Jackson ImmunoResearch AffiniPure™ Rabbit Anti-GFP is an affinity-purified polyclonal antibody raised in rabbit and can be used to detect Aequorea victoria Green Fluorescent Protein (avGFP) and its derivatives, including EGFP, ECFP, and EYFP. The antibody is suitable for use in a number of applications and is available unconjugated or conjugated to reporter enzymes, Biotin, R-PE, or Alexa Fluor® dyes.
PolyHistidine Tags (His Tags) are a popular addition to recombinant proteins to facilitate protein purification and detection. Anti-His Tag antibodies enable the detection of the tagged proteins allowing screening of expression.
Mouse Anti-Biotin, Anti-Fluorescein, Anti-Digoxin and Anti-Lactoferrin are available in a wide range of conjugate options for direct detection of their target molecules, which are commonly used as tags on proteins and nucleic acids. If signal amplification is desired, these antibodies can be used unconjugated, followed by conjugated anti-mouse IgG (H+L).
Anti-horseradish peroxidase (HRP) may be used to detect HRP or to enhance signal by binding to HRP-conjugated molecules. Anti-HRP also may be used to convert an HRP conjugate into a different signal.
