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          Biotin-SP (long spacer) AffiniPure-VHHâ„¢ Fragment Alpaca Anti-Human IgG (H+L)

          (min X Bov, Ms, Rb Sr Prot)

          Conjugate Options

          Target: Human
          Host: Alpaca
          Antibody Format: VHH Fragment
          Specificity: IgG (H+L)
          Minimal Cross Reactivity: Bovine, Mouse, Rabbit Serum Proteins
          Conjugate: Biotin-SP (long spacer)
          Product Category: AffiniPure-VHHâ„¢ Secondary Antibodies
          RRID: AB_2922852

          This product is licensed for in vitro research use only. It is not a medical device and it is not intended for diagnostic or therapeutic purposes. Product cannot be resold in any format without expressed authorization from Jackson ImmunoResearch Laboratories, Inc.
          Jackson ImmunoResearch Laboratories, Inc. has trademark rights to AffiniPure-VHHâ„¢.

          Code: 609-064-213

          Unit:
          0.1 ml
          Price:
          $239.00
          Quantity:

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          Technical Info

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          Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule human IgG. It also reacts with the light chains of other human immunoglobulins. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with bovine, mouse and rabbit serum proteins, but it may cross-react with immunoglobulins from other species.

          AffiniPure-VHH™ secondaries are polyclonal single-domain antibodies (nanobodies†) derived from hyper-immunized alpacas. 10x smaller than conventional whole IgG antibodies, <15KDa VHH fragments are perfect for imaging experiments where good penetration is necessary. AffiniPure-VHH™ secondary antibodies are cross-adsorbed for exquisite specificity against target species with minimal cross‑reactivity to other stated species, making them suitable for application in multiple labeling experiments.

          †Nanobody® is a trademark of ABLYNX N.V.
          Usage
          Physical State: Freeze-dried solid

          Storage and Rehydration: Store freeze-dried solid at 2-8°C. Rehydrate with the indicated volume of dH2O (see product specification sheet) and centrifuge if not clear. Prepare working dilution on day of use. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid.
          Extended Storage after Rehydration: Aliquot and freeze at -70°C or below. Avoid repeated freezing and thawing. Alternatively, add an equal volume of glycerol (ACS grade or better) for a final concentration of 50%, and store at -20°C as a liquid.
          Expiration date: one year from date of rehydration. The expiration date may be extended if test results are acceptable for the intended use.


          Purity: The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads. Fc fragments and whole IgG molecules have been removed.
          Buffer: 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6
          Stabilizer: 15 mg/ml Bovine Serum Albumin (IgG-Free, Protease-Free)
          Preservative: 0.05% Sodium Azide

          Suggested Working Concentration or Dilution Range:

          1:20,000 - 1:400,000 for ELISA and Western blotting using enzyme-conjugated streptavidin
          1:500 - 1:5,000 for enzyme immunohisto/cytochemistry
          1:200 - 1:1,000 for flow cytometry and fluorescence immunohisto/cytochemistry

          Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.

          Conjugate

          Biotin-SP (long spacer)



          Biotin-SP is our trade name for biotin with a 6-atom spacer positioned between biotin and the protein to which it is conjugated. When Biotin-SP-conjugated antibodies are used in enzyme immunoassays, there is an increase in sensitivity compared to biotin-conjugated antibodies without the spacer. This is especially notable when Biotin-SP conjugated antibodies are used with alkaline phosphatase-conjugated streptavidin. Apparently, the long spacer extends the biotin moiety away from the antibody surface, making it more accessible to binding sites on streptavidin. Biotinylated antibodies require an additional reagent for visualization. We offer streptavidin and Mouse Anti-Biotin conjugated to fluorophores and enzymes.

          This product is for in vitro research use only. It is not a medical device and it is not intended for diagnostic or therapeutic purposes. Product cannot be resold in any format without expressed authorization from Jackson ImmunoResearch Laboratories, Inc.


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