For immunofluorescence experiments with multiple targets, it's essential to use secondary antibodies cross-adsorbed against other species in the assay, which are conjugated to spectrally distinct fluorescent dyes. Here, we demonstrate how to combine New Alexa Fluor® 555 and 568 conjugated secondary antibodies into your four-color immunofluorescence experiments.
NEW Alexa Fluor® 555 and Alexa Fluor® 568 dyes are conjugated to Donkey and Goat host species secondary antibodies designed with multiple labeling in mind as they are minimally cross-reactive to many common species.
JIR AffiniPure-VHH™ Secondary Antibodies enable high-precision staining by combining the exquisite specificity of our minimally cross-reactive antibodies with the unique penetrative capabilities of the small VHH fragment format.
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Flow cytometry is a powerful technique for measuring and analyzing the physical characteristics of single particles in solution as they travel past a beam of light.
Immunofluorescence (IF) is an immunochemical technique that enables the detection and localization of antigens on tissue or cells using microscopy or flow cytometry. Fluorescent immunostaining allows protein expression patterns and interactions to be characterized.
