Jackson Immuno Research Inc.
specializing in secondary antibodies and conjugates
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Whole IgG Affinity-Purified Secondary Antibodies

"I have used a wide variety of secondaries and Jackson ImmunoResearch has consistently been the best. The fluorophores are bright and stable and their selective (x reactivity removed) secondaries have always shown species specificity in multiple labeling."

Janet Duerr, Ohio University

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Jackson ImmunoResearch Literature Download

You can download our product information literature via the links below. If there are any additional topics you would like us to cover, please let us know.


Flow Cytometry

Literature

Four-Color Immunofluorescence with Alexa Fluor® 555 & 568

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For immunofluorescence experiments with multiple targets, it's essential to use secondary antibodies cross-adsorbed against other species in the assay, which are conjugated to spectrally distinct fluorescent dyes. Here, we demonstrate how to combine New Alexa Fluor® 555 and 568 conjugated secondary antibodies into your four-color immunofluorescence experiments.

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NEW Alexa Fluor® 555 & 568 Secondary Antibody Conjugates

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NEW Alexa Fluor® 555 and Alexa Fluor® 568 dyes are conjugated to Donkey and Goat host species secondary antibodies designed with multiple labeling in mind as they are minimally cross-reactive to many common species.

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Control and Blocking Reagents Guide

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When developing an immunotechnique, it is important to consider result analysis. The addition of the correct blocking reagents and experimental controls can improve assay performance and interpretation.

Download

Introduction to Flow Cytometry

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Flow cytometry is a powerful technique for measuring and analyzing the physical characteristics of single particles in solution as they travel past a beam of light.

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FabuLight™ - Label Primary Antibodies in Solution

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FabuLight antibodies are Fab fragment secondary antibodies specific to the Fc region and are used to label of primary antibodies prior to incubation with cells or tissue.

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Anti-VHH Brochure

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VHH antibody (nanobody) generation can be expedited with the right tools, find out how JIR anti-VHH antibodies can help your development goals.

Download

Secondary Antibodies for VHH Discovery

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JIR presents Anti-Alpaca IgG, Subclass and VHH domain secondary antibodies to optimize VHH (single domain) antibody development and application.

Download

Secondary Antibody Conjugates for Flow Cytometry

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Fluorescent proteins. R-PE, APC, and PerCP can be used for flow-cytometry.

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PerCP Conjugates for Flow Cytometry

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PerCP is a fluorescent peridinin-chlorophyll protein complex isolated from dinoflagellates still popular for flow cytometry.

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Brilliant Violet™ 421 and 480 Conjugates

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Achieve 5-color fluorescent labeling with Brilliant Violet 421™and Brilliant Violet 480™ conjugated secondary antibodies.

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4-Color Imaging With DyLight™ 405 Conjugates

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DyLight-405-conjugated secondary antibodies are very bright and photostable when used with confocal microscopes. Add DyLight 405 to your dye panel to achieve 4-colour imaging.

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Immunolabeling

Literature

Imaging with AffiniPure-VHH™

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JIR AffiniPure-VHH™ Secondary Antibodies enable high-precision staining by combining the exquisite specificity of our minimally cross-reactive antibodies with the unique penetrative capabilities of the small VHH fragment format.

Download

Four-Color Immunofluorescence with Alexa Fluor® 555 & 568

Thumbnail Preview of Four-Color Immunofluorescence with Alexa Fluor® 555 & 568

For immunofluorescence experiments with multiple targets, it's essential to use secondary antibodies cross-adsorbed against other species in the assay, which are conjugated to spectrally distinct fluorescent dyes. Here, we demonstrate how to combine New Alexa Fluor® 555 and 568 conjugated secondary antibodies into your four-color immunofluorescence experiments.

Download

NEW Alexa Fluor® 555 & 568 Secondary Antibody Conjugates

Thumbnail Preview of Conjugates Designed for Multiple Labeling - Alexa Fluor® 555 & 568

NEW Alexa Fluor® 555 and Alexa Fluor® 568 dyes are conjugated to Donkey and Goat host species secondary antibodies designed with multiple labeling in mind as they are minimally cross-reactive to many common species.

Download

Control and Blocking Reagents Guide

Thumbnail Preview of Control and Blocking Reagents guide

When developing an immunotechnique, it is important to consider result analysis. The addition of the correct blocking reagents and experimental controls can improve assay performance and interpretation.

Download

Introduction to Immunofluorescence

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Immunofluorescence (IF) is an immunochemical technique that enables the detection and localization of antigens on tissue or cells using microscopy or flow cytometry. Fluorescent immunostaining allows protein expression patterns and interactions to be characterized.

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Introduction to Blocking and Control Reagents

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When developing an immunotechnique, it is important to consider result analysis. The addition of the correct blocking reagents and experimental controls can improve assay performance, interpretation and ultimately the presentation of data for publication.

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Introduction to AffiniPure-VHH™ Antibodies

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Jackson ImmunoResearch AffiniPure-VHH™ Secondaries are a specialized detection reagent that harnesses the unique properties of VHH Fragments to produce high-quality results.

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FabuLight™ - Label Primary Antibodies in Solution

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FabuLight antibodies are Fab fragment secondary antibodies specific to the Fc region and are used to label of primary antibodies prior to incubation with cells or tissue.

Download

Alpaca Secondary Antibodies

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Need an alternative host species? Find out how Alpaca antibodies can provide an alternative to goat or donkey secondaries, for example in a multiple labeling assay which has potential unwanted cross-reactions.

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Secondary Antibodies for Superresolution Microscopy

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Achieve the highest resolution with JIR fluorescent dye conjugates.

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Distinguish Mouse Immunoglobulin Subclasses

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Anti-Mouse IgG Subclass Specific antibodies have been extensively adsorbed against other mouse IgG subclasses enabling accurate subclass differentiation.

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Cy™2, 3, & 5 - Brighter in Plastic Mounting Media

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Cyanine dyes are a robust choice, better able to withstand the harsh dehydration and embedding conditions required for mounting sections in non-polar plastic media.

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Colloidal Gold Conjugates

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Colloidal gold reagents are available either for transmission and scanning electron microscopy (EM Grade 6, 12 & 18 nm) or for light microscopy and immunoblotting (LM Grade 4 nm).

Download

Brilliant Violet™ 421 and 480 Conjugates

Thumbnail Preview of Brilliant Violet™ 421 and 480 Conjugates

Achieve 5-color fluorescent labeling with Brilliant Violet 421™and Brilliant Violet 480™ conjugated secondary antibodies.

Download

4-Color Imaging With DyLight™ 405 Conjugates

Thumbnail Preview of 4-Color Imaging With DyLight 405 Conjugates

DyLight-405-conjugated secondary antibodies are very bright and photostable when used with confocal microscopes. Add DyLight 405 to your dye panel to achieve 4-colour imaging.

Download

Posters

Guide to Multiple Labeling with Secondary Antibodies

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Email marketing@jacksonimmuno.com to receive a hard copy poster by mail.


Western Blotting

Secondary Antibody Resources Articles

Western Blot Troubleshooting Guide

Blotting tank

Western blotting is a staple technique of the molecular biology lab. The robust nature of the antigen-antibody interaction allows the presence of specific proteins and peptides to be detected from complex mixtures. If the Western blot is not behaving as expected, our troubleshooting guide may help isolate the problem.

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Western Blotting Guide: Parts 1-8

Gloved scientist observing a western blot film

Western blotting is widely used today in molecular biology, biochemistry and cell biology to detect specific proteins of interest from biological samples. This technique provides information about the target protein(s), including abundance and molecular weight, and provides a way of detecting protein-protein interactions and post-translational modifications.

Read More

Literature

Control and Blocking Reagents Guide

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When developing an immunotechnique, it is important to consider result analysis. The addition of the correct blocking reagents and experimental controls can improve assay performance and interpretation.

Download

10 Tips for Better Blots

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Western blotting is a technique used to confirm the presence of target proteins and peptides from complex mixtures. Proteins are separated by size using PAGE, transferred to a solid support, and visualized using pairs of primary and secondary antibodies.

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Introduction to Anti-His Tag Antibodies

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The addition of PolyHistidine Tags (His Tags) to recombinant proteins is a common method to enable protein purification and detection. The His Tag is usually formed of 6 histidine residues, but may be between three and ten, usually at the C- or N-terminus of the recombinant protein.

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Light Chain Specific Antibodies for Western Blotting After Immunoprecipitation

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Visualizing proteins of 50kDa by Western blot following immunoprecipitation (IP). Use anti-light chain antibodies for clearer results.

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Far-red/Infrared Conjugates for High Sensitivity Western Blots

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AlexaFluor® 680 and 790 offer excellent sensitivity for fluorescent western blotting.

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Distinguish Mouse Immunoglobulin Subclasses

Thumbnail Preview of Distinguish Mouse Immunoglobulin Subclasses

Anti-Mouse IgG Subclass Specific antibodies have been extensively adsorbed against other mouse IgG subclasses enabling accurate subclass differentiation.

Download

Posters

Guide to Immunoglobulins Structure and Function

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Email marketing@jacksonimmuno.com to receive a hard copy poster by mail.


ELISA, LFIA and Rapid Test

Secondary Antibody Resources Articles

ELISA Guide: Parts 1-4

Gloved scientist setting up ELISA plate reader

Enzyme-linked immunosorbent assay (ELISA) was first described in 1971 when it was developed as a safer and more convenient alternative to radioimmunoassay. It has since become a widely used technique for detecting and, most importantly, quantifying one or more specific proteins within a complex mixture.

Read More

Literature

Antibodies for Point-of-Care Immunoassays

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Learn about Secondary Antibodies for Immunoassay Development and Manufacture

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Control and Blocking Reagents Guide

Thumbnail Preview of Control and Blocking Reagents guide

When developing an immunotechnique, it is important to consider result analysis. The addition of the correct blocking reagents and experimental controls can improve assay performance and interpretation.

Download

Anti-Human IgE Antibodies Flyer

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Jackson ImmunoResearch Mouse Monoclonal Anti-Human IgE antibodies complement our existing Anti-Human IgG, IgM, and IgA antibodies suitable for diagnostics research and development. All are available conjugated to a select range of reporter molecules, including Alkaline Phosphatase and Biotin enabling excellent sensitivity.

Download

Reagents for Diagnostic Immunoassays

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A complete suite of antibodies and reagents for the development and optimization of accurate and sensitive immunoassays for diagnostic serological applications.

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Secondary Antibodies for VHH Discovery

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JIR Anti-Alpaca antibodies can be utilized at specific stages to expedite and enhance the development of high affinity VHH antibodies.

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Streptavidin Conjugates for Amplification

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Streptavidin has high affinity for biotin and is used as a replacement for avidin. Available with a variety of conjugates find out how Streptavidin can enhance your assay.

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Reducing Background from Bovine Ig

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Bovine serum albumin (BSA) and dry milk are common blocking reagents, find out why JIR BSA (IgG free, Protease-free) is a better choice.

Download

Colloidal Gold Conjugates

Thumbnail Preview of Colloidal Gold Conjugates

Colloidal gold reagents are available either for transmission and scanning electron microscopy (EM Grade 6, 12 & 18 nm) or for light microscopy and immunoblotting (LM Grade 4 nm).

Download

Nanobody Development

Literature

Imaging with AffiniPure-VHH™

Thumbnail Preview of Imaging with AffiniPure-VHH

JIR AffiniPure-VHH™ Secondary Antibodies enable high-precision staining by combining the exquisite specificity of our minimally cross-reactive antibodies with the unique penetrative capabilities of the small VHH fragment format.

Download

Anti-Camelid Secondary Antibodies

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JIR offers three new secondary antibody specificities for detection and quantification of camelid antibodies.

Download

Secondary Antibodies for VHH Discovery

Thumbnail Preview of Secondary Antibodies for VHH Discovery

JIR presents Anti-Alpaca IgG, Subclass and VHH domain secondary antibodies to optimize VHH (single domain) antibody development and application.

Download

Anti-VHH Brochure

Thumbnail Preview of Anti-VHH Brochure

VHH antibody (nanobody) generation can be expedited with the right tools, find out how JIR anti-VHH antibodies can help your development goals.

Download

Commercial Applications

Literature

Reagents for Veterinary Immunoassay Development and Manufacture

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Veterinary diagnostic tests are critical in maintaining the health of livestock and companion animals, enabling the prevention and treatment of disease to ensure productivity and welfare.

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Antibodies for Custom and OEM Services

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Access our custom and bulk services for reagents used in the development and optimization of immunoassays for in-vitro diagnostics.

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Reagents for Diagnostic Immunoassays

Thumbnail Preview of Reagents for Diagnostic Immunoassays

A complete suite of antibodies and reagents for the development and optimization of accurate and sensitive immunoassays for diagnostic serological applications.

Download

Anti-Human IgE Antibodies Flyer

Thumbnail Preview of Anti-Human IgE Antibodies

Jackson ImmunoResearch Mouse Monoclonal Anti-Human IgE antibodies complement our existing Anti-Human IgG, IgM, and IgA antibodies suitable for diagnostics research and development. All are available conjugated to a select range of reporter molecules, including Alkaline Phosphatase and Biotin enabling excellent sensitivity.

Download

Secondary Antibodies and Related Reagents Catalog

To request a hard copy, fill out the form here.

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