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specializing in secondary antibodies and conjugates

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Whole IgG Antibodies
F(ab')₂ Fragment Antibodies
Fab Fragment Antibodies
AffiniPure-VHH® Antibodies
Antisera

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Alpaca
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Alpaca
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GFP
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304 products available

Conjugate Selection: (Unconjugated)

Unconjugated

Unconjugated results are shown by default, to deselect unconjugated, select another conjugate option first.

Enzymes/Biotin Select all

Horseradish Peroxidase

Alkaline Phosphatase

Biotin-SP (long spacer)

Excitation Range 350-436 nm (DyLight 405, BV421, BV480 and AMCA) Select all

DyLight™ 405

Brilliant Violet 421™

Coumarin AMCA

Brilliant Violet 480™

Excitation Range 488-493 nm (R-PE, PerCP, Cy2, Alexa Fluor 488 and FITC) Select all

Cyanine Cy™2

Alexa Fluor® 488

Fluorescein (FITC)

Fluorescein (DTAF)

R-Phycoerythrin

PerCP

Excitation Range 550-594 nm (Cy3, RR-X, Alexa Fluor 555, 568 & 594) Select all

Cyanine Cy™3

Rhodamine (TRITC)

Alexa Fluor® 555

Rhodamine Red™-X (RRX)

Alexa Fluor® 568

Alexa Fluor® 594

Texas Red®

Excitation Range 650-792 nm (APC, Cy5, Alexa Fluor 647, 680 and 790) Select all

Allophycocyanin (APC)

Alexa Fluor® 647

Cyanine Cy™5

Alexa Fluor® 680

Alexa Fluor® 790

Colloidal Gold Select all

4 nm Colloidal Gold

6 nm Colloidal Gold

12 nm Colloidal Gold

18 nm Colloidal Gold

40 nm Colloidal Gold

304 products available

Polyclonal AffiniPure-VHH™ Secondaries

Amplify the Detail

New VHH Fragment Antibodies for High-Resolution Immunostaining

Common Applications of Secondary Antibodies

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Applications

Western Blotting

Detection Methods
Western Blotting After IP
Near-Infrared Fluorescent Conjugates

Immunostaining

Tissue or Sample Specifics
Primary Antibody Choice
Selecting the Secondary Antibody for IHC and IF

Flow Cytometry

Indirect Flow Cytometry
Fluorescent Conjugates
Controls

Super-Resolution Microscopy

Stimulated Emission Depletion (STED) Microscopy
Stochastic Optical Reconstruction Microscopy (STORM)

ELISA

Comparison of ELISA Methods by Step
Blocking
Labeled Streptavidin with Biotinylated Antibodies for Enhanced Sensitivity

Electron Microscopy

Jackson ImmunoResearch ImmunoGold Secondary Antibodies for EM

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