Alexa Fluor® 488

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Alexa Fluor® 488 Technical Information

Alexa Fluor® 488 - conjugated antibodies absorb light maximally at 493 nm and fluoresce with a peak around 519 nm. In aqueous mounting media, they are brighter and more photostable than FITC, Cy™2 and DyLight™ 488. Alexa Fluor® 488 conjugates are recommended for maximum sensitivity for all immunofluorescence procedures requiring a green-fluorescing dye, except for protocols that include mounting in plastic mounting media (see Cy™2 for more information).

Alexa Fluor® 488 is a versatile dye and can be combined with different dyes for effective 4 color immunofluorescence.

Alexa Fluor® 488 is available conjugated to:

• Whole IgG Secondary Antibodies
• F(ab')₂ Fragment Secondary Antibodies
• Fab Fragment Secondary Antibodies
• FabuLight™ - Fc Specific Fab Fragments for Labeling Primary Antibodies In Solution
• Anti-Fluorescein, Anti-Digoxin, Anti-Biotin, and Anti-HRP Antibodies
• Streptavidin
• ChromPure™ Purified Proteins from Normal Serums

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Alexa Fluor® 488 Conjugated Antibodies

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Control Reagents	Product Code
Alexa Fluor® 488-conjugated ChromPure Bovine IgG, whole molecule	001-540-003
Alexa Fluor® 488-conjugated ChromPure Chicken IgY (IgG), whole molecule	003-540-003
Alexa Fluor® 488-conjugated ChromPure Donkey IgG, F(ab')2 fragment	017-540-006
Alexa Fluor® 488-conjugated ChromPure Donkey IgG, whole molecule	017-540-003
Alexa Fluor® 488-conjugated ChromPure Goat IgG, F(ab')2 fragment	005-540-006
Alexa Fluor® 488-conjugated ChromPure Goat IgG, whole molecule	005-540-003
Alexa Fluor® 488-conjugated ChromPure Human Albumin	009-540-051
Alexa Fluor® 488-conjugated ChromPure Human IgG, F(ab')2 fragment	009-540-006
Alexa Fluor® 488-conjugated ChromPure Human IgG, Fab fragment	009-540-007
Alexa Fluor® 488-conjugated ChromPure Human IgG, Fc fragment	009-540-008
Alexa Fluor® 488-conjugated ChromPure Human IgG, whole molecule	009-540-003
Alexa Fluor® 488-conjugated ChromPure Human IgM (myeloma), whole molecule	009-540-012
Alexa Fluor® 488-conjugated ChromPure Human Transferrin	009-540-050
Alexa Fluor® 488-conjugated ChromPure Mouse IgG, F(ab')2 fragment	015-540-006
Alexa Fluor® 488-conjugated ChromPure Mouse IgG, whole molecule	015-540-003
Alexa Fluor® 488-conjugated ChromPure Mouse Transferrin	015-540-050
Alexa Fluor® 488-conjugated ChromPure Rabbit IgG, whole molecule	011-540-003
Alexa Fluor® 488-conjugated ChromPure Rat IgG, whole molecule	012-540-003
Alexa Fluor® 488-conjugated ChromPure Rat Transferrin	012-540-050
Alexa Fluor® 488-conjugated ChromPure Sheep IgG, whole molecule	013-540-003
Streptavidin	Product Code
Alexa Fluor® 488-conjugated Streptavidin	016-540-084

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Spectra Viewer

Use the spectra viewer below to compare Alexa Fluor® 488 with other fluorophores, along with instrument specifics (laser/filter) for suitability in your assay.

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