"I have used a wide variety of secondaries and Jackson ImmunoResearch has consistently been the best. The fluorophores are bright and stable and their selective (x reactivity removed) secondaries have always shown species specificity in multiple labeling."
Cy™2 - conjugates have maximum absorption/excitation at 492 nm and fluoresce with a peak around 510 nm in the green region of the visible spectrum like FITC conjugates (520 nm), but they are more photostable and less sensitive to pH changes than FITC. The main advantage of Cy™2 conjugates is increased fluorescence in plastic mounting media compared with other green-fluorescing dyes.
Caution: Cy2 is sensitive to p-phenylenediamine, an anti-fading agent found in some aqueous mounting media, which results in weak and diffused fluorescence after storage of stained slides.
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