"I have used a wide variety of secondaries and Jackson ImmunoResearch has consistently been the best. The fluorophores are bright and stable and their selective (x reactivity removed) secondaries have always shown species specificity in multiple labeling."
ChromPure® Proteins (from Normal Serums) and Conjugates
ChromPure is our trade name for highly purified proteins from the serum of non-immunized animals. The purified immunoglobulins in this section do not represent antibodies directed against known antigens.
Preparation
ChromPure proteins are prepared by a variety of methods, including ion-exchange, gel-filtration, hydrophobic, dye-ligand, metal-affinity, Protein A, and immunoaffinity chromatographies. Enzyme digestion is used to generate F(ab')2 (pepsin), and Fab and Fc (papain) fragments from highly purified whole molecules.
No contaminating whole molecules or undesired fragments are observed at a protein concentration of 20 mg/ml when tested by immunoelectrophoresis against anti-whole serums, anti-immunoglobulins (class specific), or anti-fragment specific antisera.
Applications
ChromPure proteins are ideal for use as experimental controls (isotype controls).
Note: ChromPure proteins are not intended for use as immunogens to produce monospecific antibodies, nor should they be used as molecular weight markers.
Format
Unconjugated ChromPure proteins are supplied as sterile-filtered liquids without stabilizers or preservative. Conjugated ChromPure proteins are freeze-dried with stabilizers and a preservative, with the exception of peroxidase conjugates, which do not contain a preservative.
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