"I have used a wide variety of secondaries and Jackson ImmunoResearch has consistently been the best. The fluorophores are bright and stable and their selective (x reactivity removed) secondaries have always shown species specificity in multiple labeling."
Janet Duerr, Ohio UniversityRating: 5.0
Jackson ImmunoResearch continues to expand our comprehensive range of high quality secondary antibodies for the life science community.
The latest addition to our catalog is a new host species, Alpaca. This presents a new option when goat or donkey secondaries may not be preferable, for example in a multiple labeling assay which has potential unwanted cross-reactions. Secondary antibodies produced in alpaca exhibit inherent minimal cross-reactivity with camelid-derived antibodies (e.g. VHH antibodies, single domain antibodies, nanobodies†).
Alpacas are part of the camelid family, along with llamas and camels. JIR’s affinity purified polyclonal antibodies produced by alpacas are predominantly IgG1 (the conventional subclass featuring both heavy and light chains) and have binding properties that are similar to those derived from the more familiar host species. These antibodies also include the heavy chain only subclasses IgG2 and IgG3.
Learn more about camelid immunoglobulins.
JIR’s alpaca secondary antibodies are prepared to ensure minimal cross-reactivity with bovine serum proteins to avoid background from BSA blocking steps and cell culture components. Further cross-adsorptions enable multiple labeling with the other available target species as shown below.
Read more about multiple labeling protocols.
| Normal Alpaca Serum | Fill Size |
|---|---|
| 028-000-001 | 2.0 ml |
| 028-000-121 | 10.0 ml |
Learn more about the general principles of blocking and controls in our guide.
