"I have used a wide variety of secondaries and Jackson ImmunoResearch has consistently been the best. The fluorophores are bright and stable and their selective (x reactivity removed) secondaries have always shown species specificity in multiple labeling."
NEW Alexa Fluor® 555 and Alexa Fluor® 568 conjugates from Jackson ImmunoResearch
Access the full spectrum - flexible panel design
Secondary antibody conjugates designed for multiple labeling
NEW Alexa Fluor® 555 and Alexa Fluor® 568 dyes are conjugated to Donkey and Goat host species secondary antibodies designed with multiple labeling in mind as they are minimally cross-reactive to many common species. Combine Alexa Fluor® 555 and 568 with our full range of JIR Alexa Fluor® conjugated secondary antibodies to design labeling experiments that access the full spectrum!
Table 1: Alexa Fluor® dyes available conjugated to Jackson ImmunoResearch products.
Alexa Fluor® dye conjugates are the industry standard for brightness and usability. They are highly water-soluble and are fluorescent across a wide pH range. They offer superb brightness and photostability with minimal background and are compatible with the most common excitation sources. Jackson ImmunoResearch introduces two new dyes to its portfolio, Alexa Fluor® 555 and 568, offering you two more choices in the orange channel for more flexible panel design!
Figure 2: Excitation and Emission spectra for Alexa Fluor® 555 and 568
Alexa Fluor® 555 conjugated antibodies absorb light maximally around 552 nm and fluoresce with a peak around 572 nm. Alexa Fluor® 555 dye is a bright, orange-fluorescent dye with excitation ideally suited to the 555 nm laser line. Alexa Fluor® 555 dye is pH-insensitive over a wide molar range, providing stable signal generation for flow cytometry and imaging applications, including some super-resolution protocols (Goossen-Schmidt et al., 2020). Dye molecules like Alexa Fluor® 555, with high fluorescence quantum yield and high photostability, allow the detection of low-abundance biological structures with great sensitivity.
Figure 3: IHC of sagittal section of FFPE mouse brain. Neurons visualized with Rabbit anti-Tyrosine Hydroxylase followed by Alexa Fluor® 555 conjugated Goat Anti-Rabbit (111-565-144) (Yellow), Nuclei stained with Dapi (Blue). Image obtained from slides created and provided by UNC's Histology Research Core Facility.
Alexa Fluor® 568 Secondary Antibodies
Dye
Alexa Fluor® 568
Laser line (nm)
568
Common filter set
Excitation (nm)
577
Emission (nm)
602
Extinction co-efficient
1.4
Quantum Yield (Φ)
0.69
Molecular Weight (Da)
792
Applications
Microscopy
SRM
Flow Cytometry
Fluorisa
Fluorescent Western Blotting
Dye
Laser line (nm)
Excitation (nm)
Emission (nm)
Extinction co-efficient
Quantum Yield (Φ)
Molecular Weight (Da)
Applications
Alexa Fluor® 568
568
577
602
1.4
0.69
792
Microscopy, SRM, Flow Cytometry, Fluorisa, Fluorescent Western Blotting
Alexa Fluor® 568 conjugated antibodies absorb light maximally around 577 nm and fluoresce with a peak around 602 nm. Alexa Fluor® 568 dyes are bright, orange-fluorescent dyes with excitation ideally suited for the 568 nm laser line on the Ar-Kr mixed-gas laser. Alexa Fluor® 568 dyes are pH-insensitive over a wide molar range. Alexa Fluor® 568 is a bright and photostable orange-red dye that matches well with Red Fluorescent Protein (RFP) filter sets and may be used in stochastic optical reconstruction microscopy (STORM)—as a reporter in dSTORM (Goossen-Schmidt et al., 2020).
Figure 4: Indirect four-color immunostaining of Human epithelial (HEp-2) cells. Cellular proliferation visualized in green, using Rabbit A-Ki-67 followed by Alexa Fluor® 488-conjugated Donkey Anti-Rabbit (H+L) (711-545-152). Microtubules visualized in yellow, using Mouse anti-α-tubulin followed by AlexaFluor® 568 conjugated Donkey Anti-Mouse IgG (H+L) (715-575-150). Cell-to-cell contact visualized in red using Goat Anti- E-Cadherin, followed by Alexa Fluor® 647-conjugated Donkey Anti-Goat IgG (H+L) (705-605-147). Blue - DAPI Nuclear Stain.
Alexa Fluor® 555 and 568 are available conjugated to:
Alexa Fluor® fluorescent dyes are a trademark of Life Technologies Corp and are provided under an agreement between Life Technologies Corp and Jackson ImmunoResearch Laboratories, Inc and the manufacture, use, sale, or import of these dyes is sold pursuant to a license from Life Technologies Corp for use of its fluorescent dye technology.
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