"I have used a wide variety of secondaries and Jackson ImmunoResearch has consistently been the best. The fluorophores are bright and stable and their selective (x reactivity removed) secondaries have always shown species specificity in multiple labeling."
NEW Alexa Fluor® 568 conjugate from Jackson ImmunoResearch
Access the full spectrum - flexible panel design
Secondary antibody conjugates designed for multiple labeling
NEW Alexa Fluor® 555 and Alexa Fluor® 568 dyes are conjugated to Donkey and Goat host species secondary antibodies designed with multiple labeling in mind as they are minimally cross-reactive to many common species. Combine Alexa Fluor® 555 and 568 with our full range of JIR Alexa Fluor® conjugated secondary antibodies to design labeling experiments that access the full spectrum!
Table 1: Alexa Fluor® dyes available conjugated to Jackson ImmunoResearch products.
Alexa Fluor® dye conjugates are the industry standard for brightness and usability. They are highly water-soluble and are fluorescent across a wide pH range. They offer superb brightness and photostability with minimal background and are compatible with the most common excitation sources. Jackson ImmunoResearch introduces two new dyes to its portfolio, Alexa Fluor® 555 and 568, offering you two more choices in the orange channel for more flexible panel design!
Figure 2: Excitation and Emission spectra for Alexa Fluor® 555 and 568
Alexa Fluor® 568 conjugated antibodies absorb light maximally around 577 nm and fluoresce with a peak around 602 nm. Alexa Fluor® 568 dyes are bright, orange-fluorescent dyes with excitation ideally suited for the 568 nm laser line on the Ar-Kr mixed-gas laser. Alexa Fluor® 568 dyes are pH-insensitive over a wide molar range. Alexa Fluor® 568 is a bright and photostable orange-red dye that matches well with Red Fluorescent Protein (RFP) filter sets and may be used in stochastic optical reconstruction microscopy (STORM)—as a reporter in dSTORM (Goossen-Schmidt et al., 2020).
Figure 3: Indirect four-color immunostaining of Human epithelial (HEp-2) cells. Cellular proliferation visualized in green, using Rabbit A-Ki-67 followed by Alexa Fluor® 488-conjugated Donkey Anti-Rabbit (H+L) (711-545-152). Microtubules visualized in yellow, using Mouse anti-α-tubulin followed by AlexaFluor® 568 conjugated Donkey Anti-Mouse IgG (H+L) (715-575-150). Cell-to-cell contact visualized in red using Goat Anti- E-Cadherin, followed by Alexa Fluor® 647-conjugated Donkey Anti-Goat IgG (H+L) (705-605-147). Blue - DAPI Nuclear Stain.
Alexa Fluor® fluorescent dyes are a trademark of Life Technologies Corp and are provided under an agreement between Life Technologies Corp and Jackson ImmunoResearch Laboratories, Inc and the manufacture, use, sale, or import of these dyes is sold pursuant to a license from Life Technologies Corp for use of its fluorescent dye technology.
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